Fish passage through hydro turbines is a major concern wherever fish must navigate through hydropower facilities. The capability of predicting the risks to passing fish can inform the design of new and upgraded turbines leading to superior fish-passage performance and survival. The Biological Performance Assessment (BioPA) toolset, developed by researchers at Pacific Northwest National Laboratory, offers such predictive capability. The tool assimilates hydraulic field data, computational fluid dynamics (CFD) results, and laboratory fish-injury studies to estimate the probabilities that fish will encounter hazardous conditions during passage through specific regions of the turbines.